PCR vs. Vector Cloning
Vector Cloning:
- Relatively cheaper compared to PCR
- replicates entire DNA
- requires bacteria and plasmid
- can be used to insert DNA and produce protein
- requires specific R.E.
- Has practical uses in treating medical disorder (eg. somatropin for stunted growth)
PCR:
- Requires less time
- Higher probability of damage/corruption. if only one DNA is replicated improperly, every DNA strand in that set will be negatively affected.
- Only replicates DNA, cannot produce protein
- Useful for forensics; PCR only requires one intact DNA strand.
- Medical uses more centred in help with diagnosis (diagnosis of HIV, looking for HIV genome)
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