Monday, March 7, 2011

PCR vs. Vector Cloning

Vector Cloning:

  • Relatively cheaper compared to PCR
  • replicates entire DNA
  • requires bacteria and plasmid
  • can be used to insert DNA and produce protein
  • requires specific R.E. 
  • Has practical uses in treating medical disorder (eg. somatropin for stunted growth)
PCR:

  • Requires less time
  • Higher probability of damage/corruption.  if only one DNA is replicated improperly, every DNA strand in that set will be negatively affected.
  • Only replicates DNA, cannot produce protein
  • Useful for forensics; PCR only requires one intact DNA strand.
  • Medical uses more centred in help with diagnosis (diagnosis of HIV, looking for HIV genome)

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